Effect of heparin-binding oviduct specific proteins on in vitro embryo development in cattle
نویسندگان
چکیده
The aim of the present study was to evaluate the effect of exogenous heparin-binding oviduct specific proteins (HBP) on in vitro embryo development in cattle. Total protein (TP) was precipitated from cow oviduct tissue lysate by ammonium sulfate and fractionated by heparin agarose affinity column. SDS-PAGE profile of TP showed diverse molecular size bands. HBP showed bands between ~66 to ~97 kDa and unbound protein fraction (HUBP) exhibited ~45 to ~66 kDa bands. Protein fractions (TP, HBP & HUBP) were used in 3 different concentrations (1, 10 & 30 μg/mL) on in vitro embryo development. Cumulus-oocyte complexes were collected from slaughterhouse ovaries, washed 4-5 times and cultured in maturation media at 38.5°C with maximum humidity in 5% CO2 in air. After 24 h, oocytes were co-incubated with capacitated sperms in Fert-BO for 14-18 h and then transferred into embryo development media. Cleavage was checked after 40-42 h and transferred into replacement media. The results indicate that at 10 μg/mL of TP fraction used as media supplement increased the cleavage rate (71.87%±3.78, P<0.05) and blastocyst formation (29.37%±3.13, P<0.01) as compared to HBP and HUBP fraction. From this study, it can be concluded that both TP and HBP may play a major role during the process of fertilization and early embryonic development in vitro.
منابع مشابه
Purification of heparin binding oviduct specific proteins and its effect on in vitro embryo development in cattle.
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